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Acrosome markers of human sperm.
In this process, a large, mainly Golgi-derived membrane-enclosed structure, the acrosome, is formed on the anterior part of the sperm head. Our work suggests NHE8 as a candidate gene for human globozoospermia and as a possible drug target for sperm acrosomal marker enzyme contraception. We cannot explain this discrepancy, but differences in genetic background or the disruption of an additional gene in their gene trap approach 30 might possibly be invoked. The isolated membranes have been characterized by light and electron microscopy and enzyme analysis.
Isolation of the outer acrosomal membrane from bull sperm.
While the acrosomal enzymes hyaluronidase and acrosin are bound to the isolated membranes, they represent only a small percentage of the total activity and therefore do not provide reliable marker enzymes for this fraction. Sperm acrosomal marker enzyme may also play a role in shaping sperm nuclei by their acroplaxome-mediated tight interaction Genesis 28—
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